Research & Development Center for Cell TherapyResearch for cell specification

Research for cell specification

Ex:identification of factors that determine the differentiation potential of Pluripotent Stem Cell
Scientific Reports Jan 10;8(1):241

 Pluripotent Stem Cells (PSCs) have two distinct potentials: self-renewal and differentiation. In a range of experiments we found that PSCs lost and regained the differentiation potential reversibly by changing the culture media from Es8 to RFF2 when seeded in single cells on Vitronectin-N (VTN-N) coated dish (Figure 1A). To explore the molecular mechanisms that regulate the differentiation potential of PSCs, a methylation status comparison study of several PSC clones that retained (or lost) differentiation potential was conducted.

The bar graphs below photos are gene expression of designated lineages obtained with qRT-PCR sore card panel.

Figure 1

Figure 1

Figure 2

Figure 2