Research & Development Center for Cell TherapyResearch for cell specification
Research for cell specification
Ex：identification of factors that determine the differentiation potential of Pluripotent Stem Cell
Scientific Reports Jan 10;8(1):241
Pluripotent Stem Cells (PSCs) have two distinct potentials: self-renewal and differentiation. In a range of experiments we found that PSCs lost and regained the differentiation potential reversibly by changing the culture media from Es8 to RFF2 when seeded in single cells on Vitronectin-N (VTN-N) coated dish (Figure 1A). To explore the molecular mechanisms that regulate the differentiation potential of PSCs, a methylation status comparison study of several PSC clones that retained (or lost) differentiation potential was conducted.
The bar graphs below photos are gene expression of designated lineages obtained with qRT-PCR sore card panel.